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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCF7L1 Recombinant antibody, PBS Only (Capture)

TCF7L1 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240544F1

Cat no : 83588-3-PBS

Synonyms

Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCF7L1 Recombinant antibody (83588-3-RR)

    WB analysis using 83588-3-RR (same clone as 83588-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83588-3-RR (TCF7L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83588-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using TCF7L1 Recombinant antibody (83588-3-RR)

    FC experiment of A549 using 83588-3-RR (same clone as 83588-3-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug TCF7L1 Recombinant antibody (83588-3-RR, Clone:240544F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83588-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00573-1

    Cytometric bead array standard curve of MP00573-1

    Cytometric bead array standard curve of MP00573-1, TCF7L1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83588-3-PBS. Detection antibody: 83588-2-PBS. Standard: Ag34253. Range: 1.25-80 ng/mL.

  • Cytometric bead array standard curve of MP00573-2

    Cytometric bead array standard curve of MP00573-2

    Cytometric bead array standard curve of MP00573-2, TCF7L1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83588-3-PBS. Detection antibody: 83588-1-PBS. Standard: Ag34253. Range: 1.25-80 ng/mL.

  • Affinity and Kinetic Characterization of 83588-3-RR

    Affinity and Kinetic Characterization of 83588-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83588-3-RR against Human TCF7L1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83588-3-PBS targets TCF7L1 as part of a matched antibody pair:

MP00573-1: 83588-3-PBS capture and 83588-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TCF7L1 fusion protein Ag34253 相同性解析による交差性が予測される生物種
Full Name transcription factor 7-like 1 (T-cell specific, HMG-box)
Calculated molecular weight 63 kDa
GenBank accession numberBC058894
Gene symbol TCF7L1
Gene ID (NCBI) 83439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.