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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCF7L1 Recombinant antibody

TCF7L1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240544F1

Cat no : 83588-3-RR

Synonyms

Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCF7L1 Recombinant antibody (83588-3-RR)

    WB analysis using 83588-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83588-3-RR (TCF7L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using TCF7L1 Recombinant antibody (83588-3-RR)

    FC experiment of A549 using 83588-3-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug TCF7L1 Recombinant antibody (83588-3-RR, Clone:240544F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83588-3-RR

    Affinity and Kinetic Characterization of 83588-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83588-3-RR against Human TCF7L1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, NCCIT cells, NIH/3T3 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83588-3-RR is TCF7L1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TCF7L1 fusion protein Ag34253 相同性解析による交差性が予測される生物種
Full Name transcription factor 7-like 1 (T-cell specific, HMG-box)
Calculated molecular weight 63 kDa
Observed molecular weight63 kDa, 75 kDa
GenBank accession numberBC058894
Gene symbol TCF7L1
Gene ID (NCBI) 83439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis

Protocols

Product Specific Protocols
WB protocol for TCF7L1 antibody 83588-3-RRDownload protocol
FC protocol for TCF7L1 antibody 83588-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols