Validation Data Gallery
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Tested Applications
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67652 targets TCF7L1/TCF7L2 in FC (Intra) applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TCF7L1/TCF7L2 fusion protein Ag28954 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor 7-like 1 (T-cell specific, HMG-box) |
| Calculated molecular weight | 63 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC058894 |
| Gene Symbol | TCF7L1 |
| Gene ID (NCBI) | 83439 |
| RRID | AB_2934557 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HCS4 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. This antibody raised against 1-200aa of human TCF7L1 can also react with TCF7L2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 TCF7L1/TCF7L2 antibody CL488-67652 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |