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Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated TCF7L1/TCF7L2 Monocl (CL488-67652)

    FC experiment of A431 using CL488-67652

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TCF7L1/TCF7L2 (CL488-67652, Clone:2B2D12) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated TCF7L1/TCF7L2 Monoclonal antibody

TCF7L1/TCF7L2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67652
Clone No.2B2D12

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

FC (Intra)

Synonyms

TCF7L1, TCF7L2, TCF4, TCF-3, TCF3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67652 targets TCF7L1/TCF7L2 in FC (Intra) applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TCF7L1/TCF7L2 fusion protein Ag28954 相同性解析による交差性が予測される生物種
Full Name transcription factor 7-like 1 (T-cell specific, HMG-box)
Calculated molecular weight 63 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC058894
Gene Symbol TCF7L1
Gene ID (NCBI) 83439
RRIDAB_2934557
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HCS4
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. This antibody raised against 1-200aa of human TCF7L1 can also react with TCF7L2.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TCF7L1/TCF7L2 antibody CL488-67652Download protocol
Standard Protocols
Click here to view our Standard Protocols