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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCN2 Monoclonal antibody

TCN2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G6D2

Cat no : 66377-1-Ig

Synonyms

TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using TCN2 Monoclonal antibody (66377-1-Ig)

    WB analysis of HUVEC using 66377-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TCN2 Monoclonal antibody (66377-1-Ig)

    WB analysis of A549 using 66377-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHUVEC cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66377-1-Ig targets TCN2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TCN2 fusion protein Ag2825 相同性解析による交差性が予測される生物種
Full Name transcobalamin II; macrocytic anemia
Calculated molecular weight 400 aa, 44 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC011239
Gene symbol TCN2
Gene ID (NCBI) 6948
RRIDAB_2881754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)

Protocols

Product Specific Protocols
WB protocol for TCN2 antibody 66377-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols