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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCN2 Monoclonal antibody, PBS Only

TCN2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6D2

Cat no : 66377-1-PBS

Synonyms

TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using TCN2 Monoclonal antibody (66377-1-Ig)

    WB analysis of HUVEC using 66377-1-Ig (same clone as 66377-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66377-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TCN2 Monoclonal antibody (66377-1-Ig)

    WB analysis of A549 using 66377-1-Ig (same clone as 66377-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66377-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66377-1-PBS targets TCN2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TCN2 fusion protein Ag2825 相同性解析による交差性が予測される生物種
Full Name transcobalamin II; macrocytic anemia
Calculated molecular weight 400 aa, 44 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC011239
Gene symbol TCN2
Gene ID (NCBI) 6948
RRIDAB_2881754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)