CoraLite® Plus 488-conjugated TDP-43 (C-terminal) Recombinant antibody
TDP-43 (C-terminal) Recombinant Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5D17
Cat no : CL488-81350
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-81350 targets TDP-43 (C-terminal) in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TDP-43 (C-terminal) fusion protein Ag4003 相同性解析による交差性が予測される生物種 |
| Full Name | TAR DNA binding protein |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC001487 |
| Gene symbol | TDP-43 |
| Gene ID (NCBI) | 23435 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TDP-43 (C-terminal) antibody CL488-81350 | Download protocol |
| FC protocol for CL Plus 488 TDP-43 (C-terminal) antibody CL488-81350 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
