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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TDRKH Monoclonal antibody, PBS Only

TDRKH Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B1B12

Cat no : 66845-1-PBS

Synonyms

TDRD2, TDRKH, tudor and KH domain containing

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis of NCCIT using 66845-1-Ig (same clone as 66845-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis of LNCaP using 66845-1-Ig (same clone as 66845-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TDRKH Monoclonal antibody (66845-1-Ig)

    IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TDRKH Monoclonal antibody (66845-1-Ig)

    IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TDRKH Monoclonal antibody (66845-1-Ig)

    IF Staining of HepG2 using 66845-1-Ig (same clone as 66845-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66845-1-PBS targets TDRKH in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TDRKH fusion protein Ag4720 相同性解析による交差性が予測される生物種
Full Name tudor and KH domain containing
Calculated molecular weight 606 aa, 67 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC032690
Gene symbol TDRKH
Gene ID (NCBI) 11022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.