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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TDRKH Monoclonal antibody

TDRKH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B1B12

Cat no : 66845-1-Ig

Synonyms

TDRD2, TDRKH, tudor and KH domain containing

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis using 66845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis using 66845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis using 66845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis of NCCIT using 66845-1-Ig

    NCCIT cell were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using TDRKH Monoclonal antibody (66845-1-Ig)

    WB analysis of LNCaP using 66845-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TDRKH Monoclonal antibody (66845-1-Ig)

    IHC staining of mouse testis using 66845-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TDRKH Monoclonal antibody (66845-1-Ig)

    IHC staining of mouse testis using 66845-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TDRKH Monoclonal antibody (66845-1-Ig)

    IF Staining of HepG2 using 66845-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inrat brain tissue, JAR cells, NCCIT cells, LNCaP cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat testis tissue, mouse testis tissue, Rat brain
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66845-1-Ig targets TDRKH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TDRKH fusion protein Ag4720 相同性解析による交差性が予測される生物種
Full Name tudor and KH domain containing
Calculated molecular weight 606 aa, 67 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC032690
Gene symbol TDRKH
Gene ID (NCBI) 11022
RRIDAB_2882185
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

Protocols

Product Specific Protocols
WB protocol for TDRKH antibody 66845-1-IgDownload protocol
IHC protocol for TDRKH antibody 66845-1-IgDownload protocol
IF protocol for TDRKH antibody 66845-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols