CoraLite®555-conjugated TDRKH Monoclonal antibody
TDRKH Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
2B1B12
Cat no : CL555-66845
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-66845 targets TDRKH in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TDRKH fusion protein Ag4720 相同性解析による交差性が予測される生物種 |
| Full Name | tudor and KH domain containing |
| Calculated molecular weight | 606 aa, 67 kDa |
| Observed molecular weight | 67-70 kDa |
| GenBank accession number | BC032690 |
| Gene symbol | TDRKH |
| Gene ID (NCBI) | 11022 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 TDRKH antibody CL555-66845 | Download protocol |
| FC protocol for CL555 TDRKH antibody CL555-66845 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
