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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated TDRKH Monoclonal antibody

TDRKH Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2B1B12

Cat no : CL555-66845

Synonyms

TDRKH specific, Tudor domain-containing protein 2, Tudor and KH domain-containing protein, tudor and KH domain containing, TDRD2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite®555-conjugated TDRKH Monoclonal antibody (CL555-66845)

    IF Staining of U2OS using CL555-66845

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite®555 TDRKH antibody (CL555-66845, Clone: 2B1B12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®555-conjugated TDRKH Monoclonal antibody (CL555-66845)

    FC experiment of MCF-7 using CL555-66845

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human TDRKH (CL555-66845, Clone:2B1B12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66845 targets TDRKH in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TDRKH fusion protein Ag4720 相同性解析による交差性が予測される生物種
Full Name tudor and KH domain containing
Calculated molecular weight 606 aa, 67 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC032690
Gene symbol TDRKH
Gene ID (NCBI) 11022
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

Protocols

Product Specific Protocols
IF protocol for CL555 TDRKH antibody CL555-66845Download protocol
FC protocol for CL555 TDRKH antibody CL555-66845Download protocol
Standard Protocols
Click here to view our Standard Protocols