Validation Data Gallery
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Tested Applications
| Positive WB detected in | Y79 cells, L02 cells, A431 cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66077-1-Ig targets TELO2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TELO2 fusion protein Ag8834 相同性解析による交差性が予測される生物種 |
| Full Name | TEL2, telomere maintenance 2, homolog (S. cerevisiae) |
| Calculated molecular weight | 837 aa, 92 kDa |
| Observed molecular weight | 92 kDa |
| GenBank accession number | BC017188 |
| Gene Symbol | TELO2 |
| Gene ID (NCBI) | 9894 |
| RRID | AB_11182835 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y4R8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), and ultraviolet (UV). The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TELO2 antibody 66077-1-Ig | Download protocol |
| IHC protocol for TELO2 antibody 66077-1-Ig | Download protocol |
| IF protocol for TELO2 antibody 66077-1-Ig | Download protocol |
| IP protocol for TELO2 antibody 66077-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tel2. |