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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TELO2 Monoclonal antibody, PBS Only (Detector)

TELO2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H10F4

Cat no : 66077-1-PBS

Synonyms

TEL2, Protein clk-2 homolog, Protein clk 2 homolog, KIAA0683, hCLK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TELO2 Monoclonal antibody (66077-1-Ig)

    WB analysis using 66077-1-Ig (same clone as 66077-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    WB analysis of Y79 using 66077-1-Ig (same clone as 66077-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    WB analysis of L02 using 66077-1-Ig (same clone as 66077-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    WB analysis of A431 using 66077-1-Ig (same clone as 66077-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50016-1

    Sandwich ELISA standard curve of MP50016-1

    Sandwich ELISA standard curve of MP50016-1, TELO2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66077-2-PBS. Detection antibody: HRP-conjugated 66077-1-PBS. Standard: Ag8834. Range: 0.156-20 ng/mL.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    IP experiment of HepG2 using 66077-1-Ig (same clone as 66077-1-PBS)

    IP result of anti-TELO2 (IP:66077-1-Ig, 4ug; Detection:66077-1-Ig 1:500) with HepG2 cells lysate 2900ug. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TELO2 Monoclonal antibody (66077-1-Ig)

    IHC staining of human kidney using 66077-1-Ig (same clone as 66077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TELO2 Monoclonal antibody (66077-1-Ig)

    IHC staining of human kidney using 66077-1-Ig (same clone as 66077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TELO2 Monoclonal antibody (66077-1-Ig)

    IHC staining of human liver cancer using 66077-1-Ig (same clone as 66077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66077-1-Ig(TELO2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TELO2 Monoclonal antibody (66077-1-Ig)

    IHC staining of human liver cancer using 66077-1-Ig (same clone as 66077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66077-1-Ig(TELO2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    IF Staining of HepG2 using 66077-1-Ig (same clone as 66077-1-PBS)

    Immunofluorescent analysis of () fixed HepG2 cells using 66077-1-Ig (TELO2 antibody) at dilution of 1:25. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50016-1

    Cytometric bead array standard curve of MP50016-1

    Cytometric bead array standard curve of MP50016-1, TELO2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66077-2-PBS. Detection antibody: 66077-1-PBS. Standard: Ag8834. Range: 1.563-12.5 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66077-1-PBS targets TELO2 as part of a matched antibody pair:

MP50016-1: 66077-2-PBS capture and 66077-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TELO2 fusion protein Ag8834 相同性解析による交差性が予測される生物種
Full Name TEL2, telomere maintenance 2, homolog (S. cerevisiae)
Calculated molecular weight 837 aa, 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC017188
Gene symbol TELO2
Gene ID (NCBI) 9894
RRIDAB_11182835
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), and ultraviolet (UV). The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.