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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC Anti-Mouse TER-119 (TER-119)

TER-119 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

TER-119

Cat no : APC-65149

Synonyms

Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse bone marrow cells using APC Anti-Mouse TER-119 (TER-119) (APC-65149)

    FC experiment of mouse bone marrow cells using APC-65149

    1x10^6 mouse bone marrow cells were surface stained with 0.5 ug APC Anti-Mouse TER-119 (TER-119) (APC-65149, Clone:TER-119) or 0.5 ug APC Rat IgG2b Isotype Control (LTF-2) (APC-65211, Clone: LTF-2), and CoraLite® Plus 488 Anti-Mouse CD45 (30-F11) (CL488-65087, Clone: 30-F11). Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse bone marrow cells using APC Anti-Mouse TER-119 (TER-119) (APC-65149)

    FC experiment of mouse bone marrow cells using APC-65149

    1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.20 ug APC-Anti-Mouse TER-119 (APC-65149, clone TER-119) (red) or 0.20 ug APC-Rat IgG2b isotype control (blue). Cells were not fixed.

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Tested Applications

Positive FC detected inmouse bone marrow cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65149 targets TER-119 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Day-14 fetal liver cells from a C57BL/6 mouse 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 76
Gene symbol TER-119
Gene ID (NCBI) 104231
RRIDAB_2883028
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).

Protocols

Product Specific Protocols
FC protocol for APC TER-119 antibody APC-65149Download protocol
Standard Protocols
Click here to view our Standard Protocols