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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TERF2IP Polyclonal antibody

TERF2IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14595-1-AP

Synonyms

Telomeric repeat-binding factor 2-interacting protein 1, RAP1 homolog, PP8000, hRap1, DRIP5

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TERF2IP Polyclonal antibody (14595-1-AP)

    WB analysis of HEK-293 using 14595-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using TERF2IP Polyclonal antibody (14595-1-AP)

    WB analysis of HT-1080 using 14595-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TERF2IP Polyclonal antibody (14595-1-AP)

    WB analysis of K-562 using 14595-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TERF2IP Polyclonal antibody (14595-1-AP)

    WB analysis of HeLa using 14595-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using TERF2IP Polyclonal antibody (14595-1-AP)

    WB analysis of HT-1080 using 14595-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TERF2IP Polyclonal antibody (14595-1-AP)

    WB analysis of HeLa using 14595-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TERF2IP Polyclonal antibody (14595-1-AP)

    WB analysis of human placenta using 14595-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TERF2IP Polyclonal antibody (14595-1-AP)

    WB analysis of A431 using 14595-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TERF2IP Polyclonal antibody (14595-1-AP)

    IP experiment of HeLa using 14595-1-AP

    IP result of anti-TERF2IP (IP:14595-1-AP, 3ug; Detection:14595-1-AP 1:800) with HeLa cells lysate 2720ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TERF2IP Polyclonal antibody (14595-1-AP)

    IHC staining of human tonsillitis using 14595-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14595-1-AP (TERF2IP antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TERF2IP Polyclonal antibody (14595-1-AP)

    IF Staining of HeLa using 14595-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TERF2IP antibody (14595-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, HT-1080 cells, human placenta tissue, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14595-1-AP targets TERF2IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TERF2IP fusion protein Ag6130 相同性解析による交差性が予測される生物種
Full Name telomeric repeat binding factor 2, interacting protein
Calculated molecular weight 44 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC005841
Gene symbol TERF2IP
Gene ID (NCBI) 54386
RRIDAB_10642697
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871)

Protocols

Product Specific Protocols
WB protocol for TERF2IP antibody 14595-1-APDownload protocol
IHC protocol for TERF2IP antibody 14595-1-APDownload protocol
IF protocol for TERF2IP antibody 14595-1-APDownload protocol
IP protocol for TERF2IP antibody 14595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

Preferential extension of short telomeres induced by low extracellular pH.

Authors - Yuanlong Ge
View Article
humanWB

Exp Cell Res

C9ORF78 partially localizes to centromeres and plays a role in chromosome segregation.

Authors - Radhika Koranne
View Article
humanIHC

J Transl Med

A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer

Authors - Huixia Huang
View Article