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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TERF2IP Polyclonal a (CL488-14595)

    IF Staining of HeLa using CL488-14595

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TERF2IP antibody (CL488-14595) at dilution of 1:200, CL594-phalloidin (red).

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CoraLite® Plus 488-conjugated TERF2IP Polyclonal antibody

TERF2IP Polyclonal Antibody for IF/ICC
Cat No. CL488-14595

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

DRIP5, hRap1, RAP1 homolog, TERF2IP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14595 targets TERF2IP in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TERF2IP fusion protein Ag6130 相同性解析による交差性が予測される生物種
Full Name telomeric repeat binding factor 2, interacting protein
Calculated molecular weight 44 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC005841
Gene Symbol TERF2IP
Gene ID (NCBI) 54386
RRIDAB_3083960
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDQ9NYB0
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TERF2IP antibody CL488-14595Download protocol
Standard Protocols
Click here to view our Standard Protocols