Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67982 targets TEX14 in IF-P applications and shows reactivity with Human, mouse, rat, rabbit samples.
| Tested Reactivity | Human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TEX14 fusion protein Ag13143 相同性解析による交差性が予測される生物種 |
| Full Name | testis expressed 14 |
| Calculated molecular weight | 957aa,107 kDa; 1497aa,168 kDa |
| Observed molecular weight | 180 kDa |
| GenBank accession number | BC040526 |
| Gene Symbol | TEX14 |
| Gene ID (NCBI) | 56155 |
| RRID | AB_2923849 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IWB6 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TEX14 antibody CL488-67982 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |