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KD/KO Validated

TEX264 Polyclonal antibody

Name: TEX264 Polyclonal antibody
Brand: Proteintech
SKU: 25858-1-AP

TEX264 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 25858-1-AP

Synonyms

SIG11, testis expressed 264, TEX264, ZSIG11

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 25858-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 25858-1-AP
WB result of TEX264 antibody (25858-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TEX264 transfected HEK-293 cells.
WB analysis of mouse testis using 25858-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 25858-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 25858-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse testis using 25858-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25858-1-AP (TEX264 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 25858-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 25858-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of U-251 using 25858-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TEX264 antibody (25858-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 25858-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, mouse testis tissue, mouse brain tissue, HeLa cells, Neuro-2a cells
Positive IHC detected in	mouse testis tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U-251 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 8 publications below
IF	See 3 publications below

Product Information

25858-1-AP targets TEX264 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TEX264 fusion protein Ag23027 相同性解析による交差性が予測される生物種
Full Name	testis expressed 264
Calculated molecular weight	313 aa, 34 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC008742
Gene symbol	TEX264
Gene ID (NCBI)	51368
RRID	AB_2880272
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

Protocols

Product Specific Protocols
WB protocol for TEX264 antibody 25858-1-AP	Download protocol
IHC protocol for TEX264 antibody 25858-1-AP	Download protocol
IF protocol for TEX264 antibody 25858-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Autophagy ATF4 links ER stress with reticulophagy in glioblastoma cells. Authors - Svenja Zielke View Article
mouse	WB,IF	ACS Chem Neurosci Anti-Inflammatory Anthranilate Analogue Enhances Autophagy through mTOR and Promotes ER-Turnover through TEX264 during Alzheimer-Associated Neuroinflammation. Authors - Zhiyu Wang KD Validated View Article
mouse	WB	Biomedicines Enhancement of TEX264-Mediated ER-Phagy Contributes to the Therapeutic Effect of Glycycoumarin against APA Hepatotoxicity in Mice. Authors - Mingzhu Yan View Article
human	WB,IF	Mol Biol Cell Lysosome Docking to WIPI1 Rings and ER-connected Phagophores Occurs During DNAJB12 and GABARAP-Dependent Selective Autophagy of Misfolded P23H-Rhodopsin. Authors - Andrew Kennedy View Article
human	WB	Mol Biol Cell CCPG1 recognizes ER luminal proteins for selective ER-phagy Authors - Shunsuke Ishii View Article
human	WB	Food Chem Toxicol Excessive ER-phagy contributes to ochratoxin A-induced apoptosis Authors - Huiqiong Deng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TEX264 Polyclonal antibody

TEX264 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 25858-1-AP

WB analysis of HEK-293 using 25858-1-AP

WB analysis of mouse testis using 25858-1-AP

WB analysis of mouse brain using 25858-1-AP

IHC staining of mouse testis using 25858-1-AP

IHC staining of mouse testis using 25858-1-AP

IHC staining of human testis using 25858-1-AP

IHC staining of human testis using 25858-1-AP

IF Staining of U-251 using 25858-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ATF4 links ER stress with reticulophagy in glioblastoma cells.

Anti-Inflammatory Anthranilate Analogue Enhances Autophagy through mTOR and Promotes ER-Turnover through TEX264 during Alzheimer-Associated Neuroinflammation.

Enhancement of TEX264-Mediated ER-Phagy Contributes to the Therapeutic Effect of Glycycoumarin against APA Hepatotoxicity in Mice.

Lysosome Docking to WIPI1 Rings and ER-connected Phagophores Occurs During DNAJB12 and GABARAP-Dependent Selective Autophagy of Misfolded P23H-Rhodopsin.

CCPG1 recognizes ER luminal proteins for selective ER-phagy

Excessive ER-phagy contributes to ochratoxin A-induced apoptosis