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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TEX264 Polyclonal antibody

TEX264 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25858

Synonyms

ZSIG11, UNQ337/PRO536, TEX 264, Testis-expressed protein 264, TEG 264

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using CoraLite® Plus 488-conjugated TEX264 Polyclonal an (CL488-25858)

    IF Staining of U-251 using CL488-25858

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 TEX264 antibody (CL488-25858) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U-251 cells using CoraLite® Plus 488-conjugated TEX264 Polyclonal an (CL488-25858)

    FC experiment of U-251 using CL488-25858

    1x10^6 U-251 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Tex264 Polyclonal Antibody (CL488-25858)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25858 targets TEX264 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEX264 fusion protein Ag23027 相同性解析による交差性が予測される生物種
Full Name testis expressed 264
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight37 kDa
GenBank accession numberBC008742
Gene symbol TEX264
Gene ID (NCBI) 51368
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TEX264 antibody CL488-25858Download protocol
FC protocol for CL Plus 488 TEX264 antibody CL488-25858Download protocol
Standard Protocols
Click here to view our Standard Protocols