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TFEB Monoclonal antibody

TFEB Monoclonal Antibody for WB, ELISA
Cat No. 68632-1-Ig
Clone No.1C2D9

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

AlphaTFEB, bHLHe35, TCFEB, TFEB, transcription factor EB

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inSK-BR-3 cells, HepG2 cells, Saos-2 cells, Ramos cells, Raji cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68632-1-Ig targets TFEB in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TFEB fusion protein Ag24850 相同性解析による交差性が予測される生物種
Full Name transcription factor EB
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight53, 65-70 kDa
GenBank accession numberBC032448
Gene Symbol TFEB
Gene ID (NCBI) 7942
RRIDAB_3085323
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19484
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Protocols

Product Specific Protocols
WB protocol for TFEB antibody 68632-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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