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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFEB Monoclonal antibody, PBS Only (Capture)

TFEB Monoclonal Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

1C2D9

Cat no : 68632-1-PBS

Synonyms

AlphaTFEB, bHLHe35, TCFEB, TFEB, transcription factor EB

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TFEB Monoclonal antibody (68632-1-Ig)

    WB analysis using 68632-1-Ig (same clone as 68632-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68632-1-Ig (TFEB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68632-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50099-1

    Cytometric bead array standard curve of MP50099-1

    Cytometric bead array standard curve of MP50099-1, TFEB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68632-1-PBS. Detection antibody: 68632-2-PBS. Standard:Ag24850. Range: 0.195-50 ng/mL.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68632-1-PBS targets TFEB as part of a matched antibody pair:

MP50099-1: 68632-1-PBS capture and 68632-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TFEB fusion protein Ag24850 相同性解析による交差性が予測される生物種
Full Name transcription factor EB
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight53, 65-70 kDa
GenBank accession numberBC032448
Gene symbol TFEB
Gene ID (NCBI) 7942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).