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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFG Monoclonal antibody

TFG Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B5B9

Cat no : 66916-1-Ig

Synonyms

FLJ36137, Protein TFG, TF6, TFG, TRK fused gene, TRK fused gene protein, TRKT3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFG Monoclonal antibody (66916-1-Ig)

    WB analysis using 66916-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TFG Monoclonal antibody (66916-1-Ig)

    WB analysis of A549 using 66916-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TFG Monoclonal antibody (66916-1-Ig)

    WB analysis of MCF-7 using 66916-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TFG Monoclonal antibody (66916-1-Ig)

    WB analysis of HEK-293 using 66916-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFG Monoclonal antibody (66916-1-Ig)

    IHC staining of human breast cancer using 66916-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFG Monoclonal antibody (66916-1-Ig)

    IHC staining of human breast cancer using 66916-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TFG Monoclonal antibody (66916-1-Ig)

    IHC staining of human prostate cancer using 66916-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TFG Monoclonal antibody (66916-1-Ig)

    IHC staining of human prostate cancer using 66916-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inNCI-H1299 cells, A549 cells, MCF-7 cells, HEK-293 cells, PC-3 cells, LNCaP cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66916-1-Ig targets TFG in WB, IHC, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TFG fusion protein Ag27697 相同性解析による交差性が予測される生物種
Full Name TRK-fused gene
Calculated molecular weight 400 aa, 43 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC023599
Gene symbol TFG
Gene ID (NCBI) 10342
RRIDAB_2882243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein TFG (TRK-fused gene protein) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. Mutations in TFG may have important clinical relevance for current therapeutic strategies to treat metastatic melanoma. Defects in TFG are a cause of thyroid papillary carcinoma (TPC), a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Hereditary motor and sensory neuropathy with proximal dominant involvement (HMSN-P) is an autosomal-dominant neurodegenerative disorder characterized by widespread fasciculations, proximal-predominant muscle weakness, and atrophy followed by distal sensory involvement. Recent genetic investigation indicates that formation of TFG-containing cytoplasmic inclusions and concomitant mislocalization of TAR DNA-binding protein 43 kDa (TDP-43) underlie motor neuron degeneration in HMSN-P. Pathological overlap of proteinopathies involving TFG and TDP-43 highlights a new pathway leading to motor neuron degeneration.

Protocols

Product Specific Protocols
WB protocol for TFG antibody 66916-1-IgDownload protocol
IHC protocol for TFG antibody 66916-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols