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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFG Monoclonal antibody, PBS Only

TFG Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5B9

Cat no : 66916-1-PBS

Synonyms

FLJ36137, Protein TFG, TF6, TFG, TRK fused gene, TRK fused gene protein, TRKT3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFG Monoclonal antibody (66916-1-Ig)

    WB analysis using 66916-1-Ig (same clone as 66916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TFG Monoclonal antibody (66916-1-Ig)

    WB analysis of A549 using 66916-1-Ig (same clone as 66916-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using TFG Monoclonal antibody (66916-1-Ig)

    WB analysis of MCF-7 using 66916-1-Ig (same clone as 66916-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TFG Monoclonal antibody (66916-1-Ig)

    WB analysis of HEK-293 using 66916-1-Ig (same clone as 66916-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFG Monoclonal antibody (66916-1-Ig)

    IHC staining of human breast cancer using 66916-1-Ig (same clone as 66916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFG Monoclonal antibody (66916-1-Ig)

    IHC staining of human breast cancer using 66916-1-Ig (same clone as 66916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TFG Monoclonal antibody (66916-1-Ig)

    IHC staining of human prostate cancer using 66916-1-Ig (same clone as 66916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TFG Monoclonal antibody (66916-1-Ig)

    IHC staining of human prostate cancer using 66916-1-Ig (same clone as 66916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66916-1-PBS targets TFG in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TFG fusion protein Ag27697 相同性解析による交差性が予測される生物種
Full Name TRK-fused gene
Calculated molecular weight 400 aa, 43 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC023599
Gene symbol TFG
Gene ID (NCBI) 10342
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein TFG (TRK-fused gene protein) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. Mutations in TFG may have important clinical relevance for current therapeutic strategies to treat metastatic melanoma. Defects in TFG are a cause of thyroid papillary carcinoma (TPC), a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Hereditary motor and sensory neuropathy with proximal dominant involvement (HMSN-P) is an autosomal-dominant neurodegenerative disorder characterized by widespread fasciculations, proximal-predominant muscle weakness, and atrophy followed by distal sensory involvement. Recent genetic investigation indicates that formation of TFG-containing cytoplasmic inclusions and concomitant mislocalization of TAR DNA-binding protein 43 kDa (TDP-43) underlie motor neuron degeneration in HMSN-P. Pathological overlap of proteinopathies involving TFG and TDP-43 highlights a new pathway leading to motor neuron degeneration.