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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated TFIP11 Monoclonal antibody (CL594-66114)

    IF Staining of HeLa using CL594-66114

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66114 (TFIP11 antibody) at dilution of 1:100.

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CoraLite®594-conjugated TFIP11 Monoclonal antibody

TFIP11 Monoclonal Antibody for IF/ICC
Cat No. CL594-66114
Clone No.1A1C8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Synonyms

bK445C9.6, FLJ22086, hNtr1, NTR1, STIP, STIP 1, TFIP11, TIP39

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66114 targets TFIP11 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TFIP11 fusion protein Ag5821 相同性解析による交差性が予測される生物種
Full Name tuftelin interacting protein 11
Calculated molecular weight 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC033080
Gene Symbol TFIP11
Gene ID (NCBI) 24144
RRIDAB_2883515
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBB9
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.

Protocols

Product Specific Protocols
IF protocol for CL594 TFIP11 antibody CL594-66114Download protocol
Standard Protocols
Click here to view our Standard Protocols