CoraLite® Plus 750-conjugated TGFB1 Recombinant antibody
TGFB1 Recombinant Antibody for WB, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, FC (Intra)
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
230544B7
Cat no : CL750-81746-2
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | 4T1 cells, RAW 264.7 cells |
Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL750-81746-2 is TGFB1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | TGFB1 fusion protein Ag13591 相同性解析による交差性が予測される生物種 |
Full Name | transforming growth factor, beta 1 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 44 kDa |
GenBank accession number | BC000125 |
Gene symbol | TGFB1 |
Gene ID (NCBI) | 7040 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1). Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 750 TGFB1 antibody CL750-81746-2 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |