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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TGM1 Monoclonal antibody

TGM1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D9C11

Cat no : 67004-1-Ig

Synonyms

Epidermal TGase, ICR2, KTG, LI, LI1, TG(K), TGASE, TGase 1, TGase K, TGK, TGM1, Transglutaminase 1, Transglutaminase K

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using TGM1 Monoclonal antibody (67004-1-Ig)

    WB analysis of human saliva using 67004-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 67004-1-Ig (TGM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TGM1 Monoclonal antibody (67004-1-Ig)

    IHC staining of human tonsillitis using 67004-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67004-1-Ig (TGM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TGM1 Monoclonal antibody (67004-1-Ig)

    IHC staining of human cervical cancer using 67004-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67004-1-Ig (TGM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman saliva tissue
Positive IHC detected inhuman tonsillitis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67004-1-Ig targets TGM1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TGM1 fusion protein Ag3905 相同性解析による交差性が予測される生物種
Full Name transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
Calculated molecular weight 817 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC034699
Gene symbol TGM1
Gene ID (NCBI) 7051
RRIDAB_2882321
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Transglutaminase 1 is a epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelope in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.

Protocols

Product Specific Protocols
WB protocol for TGM1 antibody 67004-1-IgDownload protocol
IHC protocol for TGM1 antibody 67004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Carcinogenesis

Growth modulatory effects of fenretinide encompass keratinocyte terminal differentiation: A favorable outcome for oral squamous cell carcinoma chemoprevention

Authors - Daren Wang
View Article