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Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using TGM1 Monoclonal antibody (67004-1-Ig)

    WB analysis of human saliva using 67004-1-Ig (same clone as 67004-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 67004-1-Ig (TGM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TGM1 Monoclonal antibody (67004-1-Ig)

    IHC staining of human tonsillitis using 67004-1-Ig (same clone as 67004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67004-1-Ig (TGM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TGM1 Monoclonal antibody (67004-1-Ig)

    IHC staining of human cervical cancer using 67004-1-Ig (same clone as 67004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67004-1-Ig (TGM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67004-1-PBS in a different storage buffer formulation.

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TGM1 Monoclonal antibody, PBS Only

TGM1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67004-1-PBS
Clone No.3D9C11

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

Epidermal TGase, ICR2, KTG, LI, LI1, TG(K), TGASE, TGase 1, TGase K, TGK, TGM1, Transglutaminase 1, Transglutaminase K

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67004-1-PBS targets TGM1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TGM1 fusion protein Ag3905 相同性解析による交差性が予測される生物種
Full Name transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
Calculated molecular weight 817 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC034699
Gene Symbol TGM1
Gene ID (NCBI) 7051
RRIDAB_2882321
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22735
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Transglutaminase 1 is a epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelope in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.