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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TGN46 Monoclonal antibody, PBS Only

TGN46 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F6D5

Cat no : 66477-1-PBS

Synonyms

Golgi marker, TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TGN46 Monoclonal antibody (66477-1-Ig)

    WB analysis of HeLa using 66477-1-Ig (same clone as 66477-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TGN46 Monoclonal antibody (66477-1-Ig)

    WB analysis using 66477-1-Ig (same clone as 66477-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human liver cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human liver cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66477-1-PBS targets TGN46 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.

Tested Reactivity Human, Rat, Pig, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TGN46 fusion protein Ag4470 相同性解析による交差性が予測される生物種
Full Name trans-golgi network protein 2
Calculated molecular weight 447 aa, 47 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC028219
Gene symbol TGN46
Gene ID (NCBI) 10618
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.