Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, LNCaP cells, HepG2 cells, L02 cells, SMMC-7721 cells, human colostrum tissue, pig liver tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66477-1-Ig targets TGN46 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TGN46 fusion protein Ag4470 相同性解析による交差性が予測される生物種 |
| Full Name | trans-golgi network protein 2 |
| Calculated molecular weight | 447 aa, 47 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC028219 |
| Gene Symbol | TGN46 |
| Gene ID (NCBI) | 10618 |
| RRID | AB_2881843 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43493 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TGN46 antibody 66477-1-Ig | Download protocol |
| IHC protocol for TGN46 antibody 66477-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Arterioscler Thromb Vasc Biol IL-1β Induces LDL Transcytosis by a Novel Pathway Involving LDLR and Rab27a | ||
J Virol Porcine deltacoronavirus accessory protein NS6 harnesses VPS35-mediated retrograde trafficking to facilitate efficient viral infection | ||
Int J Biol Sci Annexin A5 Derived from Cell-free Fat Extract Attenuates Osteoarthritis via Macrophage Regulation |