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TH Monoclonal antibody
TH Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2H7B7
Cat no : 66334-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-12 cells, SH-SY5Y cells |
Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:3000-1:20000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 14 publications below |
IHC | See 6 publications below |
IF | See 9 publications below |
Product Information
66334-1-Ig targets TH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TH fusion protein Ag23075 相同性解析による交差性が予測される生物種 |
Full Name | tyrosine hydroxylase |
Calculated molecular weight | 528 aa, 59 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC104967 |
Gene symbol | TH |
Gene ID (NCBI) | 7054 |
RRID | AB_2881714 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TH antibody 66334-1-Ig | Download protocol |
IF protocol for TH antibody 66334-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Ginseng Res The necessity of eliminating the interference of panaxatriol saponins to maximize the preventive effect of panaxadiol saponins against Parkinson's disease in rats | ||
Front Cell Dev Biol RAB39B Deficiency Impairs Learning and Memory Partially Through Compromising Autophagy. | ||
Antioxidants (Basel) Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency | ||
Bone Joint Res High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice | ||
Biomed Pharmacother Nardosinone regulates the slc38a2 gene to alleviate Parkinson's symptoms in rats through the GABAergic synaptic and cAMP pathways. |