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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using TH Monoclonal antibody (66334-1-Ig)

    WB analysis of PC-12 using 66334-1-Ig (same clone as 66334-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using TH Monoclonal antibody (66334-1-Ig)

    WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using TH Monoclonal antibody (66334-1-Ig)

    WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    WB result of TH antibody (66334-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TH transfected SH-SY5Y cells. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using TH Monoclonal antibody (66334-1-Ig)

    WB analysis of PC-12 using 66334-1-Ig (same clone as 66334-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using TH Monoclonal antibody (66334-1-Ig)

    IF Staining of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66334-1-Ig(TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TH Monoclonal antibody, PBS Only

TH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66334-1-PBS
Clone No.2H7B7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

tyrosine hydroxylase, Tyrosine 3 hydroxylase, TYH, EC:1.14.16.2, DYT5b

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66334-1-PBS targets TH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TH fusion protein Ag23075 相同性解析による交差性が予測される生物種
Full Name tyrosine hydroxylase
Calculated molecular weight 528 aa, 59 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC104967
Gene Symbol TH
Gene ID (NCBI) 7054
RRIDAB_2881714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07101
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.