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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD141/Thrombomodulin Monoclonal antibody

CD141/Thrombomodulin Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5A4C5

Cat no : CL488-67831

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite® Plus 488-conjugated THBD Monoclonal anti (CL488-67831)

    IF Staining of human lung cancer using CL488-67831

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 THBD antibody (CL488-67831, Clone: 5A4C5 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite® Plus 488-conjugated THBD Monoclonal anti (CL488-67831)

    IF Staining of human lung cancer using CL488-67831

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 THBD antibody (CL488-67831, Clone: 5A4C5 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman lung cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67831 targets CD141/Thrombomodulin in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD141/Thrombomodulin fusion protein Ag5723 相同性解析による交差性が予測される生物種
Full Name thrombomodulin
Calculated molecular weight 60 kDa
Observed molecular weight105 kDa
GenBank accession numberBC035602
Gene symbol THBD
Gene ID (NCBI) 7056
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD141/Thrombomodulin antibody CL488-67831Download protocol
Standard Protocols
Click here to view our Standard Protocols