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CoraLite® Plus 488-conjugated TIMELESS Monoclonal antibody
TIMELESS Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B6G3
Cat no : CL488-67022
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | HEK-293 cells |
Positive FC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67022 targets TIMELESS in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TIMELESS fusion protein Ag6283 相同性解析による交差性が予測される生物種 |
Full Name | timeless homolog (Drosophila) |
Calculated molecular weight | 139 kDa |
Observed molecular weight | 140 kDa |
GenBank accession number | BC050557 |
Gene symbol | TIMELESS |
Gene ID (NCBI) | 8914 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 TIMELESS antibody CL488-67022 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |