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  • KD/KO Validated

CoraLite® Plus 488-conjugated TIMM44 Monoclonal antibody

TIMM44 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4G7D4

Cat no : CL488-66149

Synonyms

TIMM 44, TIM44, MIMT44, 4G7D4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TIMM44 Monoclonal an (CL488-66149)

    IF Staining of HepG2 using CL488-66149

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TIMM44 antibody (CL488-66149, Clone: 4G7D4 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66149 targets TIMM44 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen TIMM44 fusion protein Ag21389 相同性解析による交差性が予測される生物種
Full Name translocase of inner mitochondrial membrane 44 homolog (yeast)
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC033628
Gene symbol TIMM44
Gene ID (NCBI) 10469
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Translocase of inner mitochondrial membrane 44 (TIMM44), also known as MIMT44 and TIM44, belongs to the Tim44 family. TIMM44 is a mitochondrial protein located at the inner mitochondrial membrane, which is crucial for the integrity and function of mitochondria. In an ATP-dependent manner, TIMM44 anchors mitochondrial heat shock protein 70 to the translocase of the mitochondrial inner membrane 23 (TIMM23) complex. TIMM44 is essential for mitochondrial pre-protein import into the mitochondrial matrix. TIMM44 is vital for the integrity and function of mitochondria. TIMM44 silencing disrupted mitochondrial functions in endothelial cells, causing mitochondrial protein input arrest, ATP reduction, ROS production, and mitochondrial depolarization, and leading to apoptosis activation (PMID: 36438483, PMID: 37147302, PMID: 38467612). The observed molecular weight of TIMM44 is 45 kDa, indicating a mature form of TIMM44 (PMID: 33891006).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TIMM44 antibody CL488-66149Download protocol
Standard Protocols
Click here to view our Standard Protocols