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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIMP1 Recombinant antibody, PBS Only

TIMP1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230329E7

Cat no : 83151-5-PBS

Synonyms

TIMP-1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, Collagenase inhibitor

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TIMP1 Recombinant antibody (83151-5-RR)

    IF Staining of HeLa using 83151-5-RR (same clone as 83151-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TIMP1 Recombinant antibody (83151-5-RR)

    IF Staining of HeLa using 83151-5-RR (same clone as 83151-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using TIMP1 Recombinant antibody (83151-5-RR)

    FC experiment of HT-29 using 83151-5-RR (same clone as 83151-5-PBS)

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MDA-MB-231 cells using TIMP1 Recombinant antibody (83151-5-RR)

    FC experiment of MDA-MB-231 using 83151-5-RR (same clone as 83151-5-PBS)

    1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83151-5-RR

    Affinity and Kinetic Characterization of 83151-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83151-5-RR against Human TIMP1 were performed. The affinity constant is 1.33 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83151-5-PBS is TIMP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name TIMP metallopeptidase inhibitor 1
Calculated molecular weight 23 kDa
GenBank accession numberBC000866
Gene symbol TIMP1
Gene ID (NCBI) 7076
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.