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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIMP1 Recombinant antibody

TIMP1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230329E7

Cat no : 83151-5-RR

Synonyms

TIMP-1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, Collagenase inhibitor

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TIMP1 Recombinant antibody (83151-5-RR)

    IF Staining of HeLa using 83151-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TIMP1 Recombinant antibody (83151-5-RR)

    IF Staining of HeLa using 83151-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of HT-29 cells using TIMP1 Recombinant antibody (83151-5-RR)

    FC experiment of HT-29 using 83151-5-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of MDA-MB-231 cells using TIMP1 Recombinant antibody (83151-5-RR)

    FC experiment of MDA-MB-231 using 83151-5-RR

    1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83151-5-RR

    Affinity and Kinetic Characterization of 83151-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83151-5-RR against Human TIMP1 were performed. The affinity constant is 1.33 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHT-29 cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83151-5-RR is TIMP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name TIMP metallopeptidase inhibitor 1
Calculated molecular weight 23 kDa
GenBank accession numberBC000866
Gene symbol TIMP1
Gene ID (NCBI) 7076
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.

Protocols

Product Specific Protocols
IF protocol for TIMP1 antibody 83151-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols