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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TINAGL1 Polyclonal antibody (12077-1-AP)

    WB analysis using 12077-1-AP (same clone as 12077-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using TINAGL1 Polyclonal antibody (12077-1-AP)

    WB analysis of human placenta using 12077-1-AP (same clone as 12077-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adrenal gland tissue using TINAGL1 Polyclonal antibody (12077-1-AP)

    WB analysis of human adrenal gland using 12077-1-AP (same clone as 12077-1-PBS)

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using TINAGL1 Polyclonal antibody (12077-1-AP)

    IP experiment of mouse heart using 12077-1-AP (same clone as 12077-1-PBS)

    IP result of anti-TINAGL1 (IP:12077-1-AP, 4ug; Detection:12077-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TINAGL1 Polyclonal antibody (12077-1-AP)

    IHC staining of human liver cancer using 12077-1-AP (same clone as 12077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TINAGL1 Polyclonal antibody (12077-1-AP)

    IHC staining of human liver cancer using 12077-1-AP (same clone as 12077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using TINAGL1 Polyclonal antibody (12077-1-AP)

    IF Staining of human liver cancer using 12077-1-AP (same clone as 12077-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TINAGL1 antibody (12077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TINAGL1 Polyclonal antibody (12077-1-AP)

    IF Staining of HepG2 using 12077-1-AP (same clone as 12077-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TINAGL1 antibody (12077-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TINAGL1 Polyclonal antibody, PBS Only

TINAGL1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 12077-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

PP6614, Oxidized LDL-responsive gene 2 protein, OLRG-2, LCN7, Glucocorticoid-inducible protein 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12077-1-PBS targets TINAGL1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TINAGL1 fusion protein Ag2721 相同性解析による交差性が予測される生物種
Full Name tubulointerstitial nephritis antigen-like 1
Calculated molecular weight 467 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC009048
Gene Symbol TINAGL1
Gene ID (NCBI) 64129
RRIDAB_2058942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZM7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Tubulointerstitial nephritis antigen-like 1 (Tinagl1), a secreted extracellular protein, was initially identified as a putative component of the ECM. Tinagl1 is most commonly associated with embryonic development, including the heart and adrenal glands. Low levels of Tinagl1 expression can be dire for craniofacial development and for female fertility.