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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIP47 Monoclonal antibody

TIP47 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4C11B1

Cat no : 66523-1-Ig

Synonyms

M6PRBP1, Perilipin 3, Mannose-6-phosphate receptor-binding protein 1, 4C11B1, 47 kDa MPR-binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIP47 Monoclonal antibody (66523-1-Ig)

    WB analysis using 66523-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of U2OS cells using TIP47 Monoclonal antibody (66523-1-Ig)

    WB analysis of U2OS using 66523-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TIP47 Monoclonal antibody (66523-1-Ig)

    IHC staining of mouse liver using 66523-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TIP47 Monoclonal antibody (66523-1-Ig)

    IHC staining of mouse liver using 66523-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TIP47 Monoclonal antibody (66523-1-Ig)

    IF Staining of HeLa using 66523-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The F-actin was stained with CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TIP47 Monoclonal antibody (66523-1-Ig)

    IF Staining of HeLa using 66523-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, U2OS cells, A549 cells, A431 cells, LNCaP cells, K-562 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inoleic acid treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66523-1-Ig targets TIP47 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIP47 fusion protein Ag1028 相同性解析による交差性が予測される生物種
Full Name mannose-6-phosphate receptor binding protein 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC007566
Gene symbol TIP47/PLIN3
Gene ID (NCBI) 10226
RRIDAB_2881886
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Protocols

Product Specific Protocols
WB protocol for TIP47 antibody 66523-1-IgDownload protocol
IHC protocol for TIP47 antibody 66523-1-IgDownload protocol
IF protocol for TIP47 antibody 66523-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Neurosci

Lipid-accumulated reactive astrocytes promote disease progression in epilepsy

Authors - Zhang-Peng Chen
View Article