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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Perilipin 3/TIP47 Monoclonal antibody (66523-1-Ig)

    WB analysis using 66523-1-Ig (same clone as 66523-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using Perilipin 3/TIP47 Monoclonal antibody (66523-1-Ig)

    WB analysis of U2OS using 66523-1-Ig (same clone as 66523-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Perilipin 3/TIP47 Monoclonal antibody (66523-1-Ig)

    IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Perilipin 3/TIP47 Monoclonal antibody (66523-1-Ig)

    IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Perilipin 3/TIP47 Monoclonal antibody (66523-1-Ig)

    IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Perilipin 3/TIP47 Monoclonal antibody (66523-1-Ig)

    IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

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Perilipin 3/TIP47 Monoclonal antibody, PBS Only

Perilipin 3/TIP47 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66523-1-PBS
Clone No.4C11B1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

M6PRBP1, Perilipin 3, TIP47, Mannose-6-phosphate receptor-binding protein 1, 4C11B1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66523-1-PBS targets Perilipin 3/TIP47 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Perilipin 3/TIP47 fusion protein Ag1028 相同性解析による交差性が予測される生物種
Full Name mannose-6-phosphate receptor binding protein 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC007566
Gene Symbol TIP47/PLIN3
Gene ID (NCBI) 10226
RRIDAB_2881886
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60664
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.