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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TJAP1 Monoclonal antibody

TJAP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1F10

Cat no : 68495-1-Ig

Synonyms

DKFZp686F06131, PILT, Tight junction protein 4, TJAP1, TJP4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TJAP1 Monoclonal antibody (68495-1-Ig)

    WB analysis using 68495-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using TJAP1 Monoclonal antibody (68495-1-Ig)

    WB analysis of HepG2 using 68495-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, HEK-293 cells, MDA-MB-231 cells, HSC-T6 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68495-1-Ig targets TJAP1 in WB, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TJAP1 fusion protein Ag11807 相同性解析による交差性が予測される生物種
Full Name tight junction associated protein 1 (peripheral)
Calculated molecular weight 547 aa, 61 kDa
Observed molecular weight 86-95 kDa
GenBank accession numberBC064401
Gene symbol TJAP1
Gene ID (NCBI) 93643
RRIDAB_3085206
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TJAP1, also named as PILT and TJP4, is colocalized at the tight junctions. TJAP1 has some forms with MW 60 kDa (native) and 86-90 kDa (modified).

Protocols

Product Specific Protocols
WB protocol for TJAP1 antibody 68495-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols