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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TJAP1 Monoclonal antibody, PBS Only

TJAP1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H1F10

Cat no : 68495-1-PBS

Synonyms

DKFZp686F06131, PILT, Tight junction protein 4, TJAP1, TJP4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TJAP1 Monoclonal antibody (68495-1-Ig)

    WB analysis using 68495-1-Ig (same clone as 68495-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68495-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TJAP1 Monoclonal antibody (68495-1-Ig)

    WB analysis of HepG2 using 68495-1-Ig (same clone as 68495-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68495-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68495-1-PBS targets TJAP1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TJAP1 fusion protein Ag11807 相同性解析による交差性が予測される生物種
Full Name tight junction associated protein 1 (peripheral)
Calculated molecular weight 547 aa, 61 kDa
Observed molecular weight 86-95 kDa
GenBank accession numberBC064401
Gene symbol TJAP1
Gene ID (NCBI) 93643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TJAP1, also named as PILT and TJP4, is colocalized at the tight junctions. TJAP1 has some forms with MW 60 kDa (native) and 86-90 kDa (modified).