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TLE1 Monoclonal antibody

TLE1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F2H11

Cat no : 66216-1-Ig

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TLE1 Monoclonal antibody (66216-1-Ig)

    WB analysis of HEK-293 using 66216-1-Ig

    WB result of TLE1 antibody (66216-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLE1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of Jurkat cells using TLE1 Monoclonal antibody (66216-1-Ig)

    WB analysis of Jurkat using 66216-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66216-1-Ig (TLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TLE1 Monoclonal antibody (66216-1-Ig)

    IHC staining of human gliomas using 66216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TLE1 Monoclonal antibody (66216-1-Ig)

    IHC staining of human gliomas using 66216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66216-1-Ig targets TLE1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TLE1 fusion protein Ag21327 相同性解析による交差性が予測される生物種
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Calculated molecular weight 83 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC010100
Gene symbol TLE1
Gene ID (NCBI) 7088
RRIDAB_2881607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).

Protocols

Product Specific Protocols
WB protocol for TLE1 antibody 66216-1-IgDownload protocol
IHC protocol for TLE1 antibody 66216-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols