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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TLE1 Monoclonal antibody (66216-1-Ig)

    WB analysis of HEK-293 using 66216-1-Ig (same clone as 66216-1-PBS)

    WB result of TLE1 antibody (66216-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLE1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TLE1 Monoclonal antibody (66216-1-Ig)

    WB analysis of Jurkat using 66216-1-Ig (same clone as 66216-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66216-1-Ig (TLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TLE1 Monoclonal antibody (66216-1-Ig)

    IHC staining of human gliomas using 66216-1-Ig (same clone as 66216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TLE1 Monoclonal antibody (66216-1-Ig)

    IHC staining of human gliomas using 66216-1-Ig (same clone as 66216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TLE1 Monoclonal antibody (66216-1-Ig)

    IHC staining of human tonsillitis using 66216-1-Ig (same clone as 66216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66216-1-Ig (TLE1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using TLE1 Monoclonal antibody (66216-1-Ig)

    IF Staining of SH-SY5Y using 66216-1-Ig (same clone as 66216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TLE1 antibody (66216-1-Ig, Clone: 2F2H11 ) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TLE1 Monoclonal antibody, PBS Only

TLE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66216-1-PBS
Clone No.2F2H11

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Transducin-like enhancer protein 1, GRG1, Enhancer of split groucho-like protein 1, 2F2H11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66216-1-PBS targets TLE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TLE1 fusion protein Ag21327 相同性解析による交差性が予測される生物種
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Calculated molecular weight 83 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC010100
Gene Symbol TLE1
Gene ID (NCBI) 7088
RRIDAB_2881607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04724
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).