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  • Featured Product
  • KD/KO Validated

Talin-1 Polyclonal antibody

Talin-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 14168-1-AP

Synonyms

TLN1, TLN, talin1, talin 1, KIAA1027

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Talin-1 Polyclonal antibody (14168-1-AP)

    WB analysis using 14168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Talin-1 Polyclonal antibody (14168-1-AP)

    WB analysis using 14168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Talin-1 Polyclonal antibody (14168-1-AP)

    IP experiment of HeLa using 14168-1-AP

    IP result of anti-Talin-1 (IP:14168-1-AP, 4ug; Detection:14168-1-AP 1:40000) with HeLa cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using Talin-1 Polyclonal antibody (14168-1-AP)

    IHC staining of human colon using 14168-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Talin-1 Polyclonal antibody (14168-1-AP)

    IHC staining of human liver cancer using 14168-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Talin-1 Polyclonal antibody (14168-1-AP)

    IHC staining of human liver cancer using 14168-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Talin-1 Polyclonal antibody (14168-1-AP)

    IF Staining of HUVEC using 14168-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Polyclonal antibody (14168-1-AP)

    IF Staining of A549 using 14168-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Polyclonal antibody (14168-1-AP)

    IF Staining of A549 using 14168-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Talin-1 Polyclonal antibody (14168-1-AP)

    IF Staining of HUVEC using 14168-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Talin-1 Polyclonal antibody (14168-1-AP)

    IF Staining of HeLa using 14168-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Talin-1 Polyclonal antibody (14168-1-AP)

    IF Staining of HepG2 using 14168-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Talin-1 Polyclonal antibody (14168-1-AP)

    IF Staining of HepG2 using 14168-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Talin-1 Polyclonal antibody (14168-1-AP)

    FC experiment of HeLa using 14168-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Talin-1 Polyclonal antibody (14168-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHUVEC cells, HeLa cells, NIH/3T3 cells, C6 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells, HepG2 cells, A549 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14168-1-AP targets Talin-1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Talin-1 fusion protein Ag5367 相同性解析による交差性が予測される生物種
Full Name talin 1
Calculated molecular weight 2541 aa, 270 kDa
Observed molecular weight 250-270 kDa
GenBank accession numberBC042923
Gene symbol Talin-1
Gene ID (NCBI) 7094
RRIDAB_2271861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Protocols

Product Specific Protocols
WB protocol for Talin-1 antibody 14168-1-APDownload protocol
IHC protocol for Talin-1 antibody 14168-1-APDownload protocol
IF protocol for Talin-1 antibody 14168-1-APDownload protocol
IP protocol for Talin-1 antibody 14168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

ACS Nano

Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes.

Authors - Xiao Huang
View Article
humanWB,IHC,IF,IP

Elife

Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.

Authors - Yixiao Zhang
  • KD Validated
View Article
humanIF

Mol Oncol

ITIH5 as a multifaceted player in pancreatic cancer suppression, impairing tyrosine kinase signaling, cell adhesion and migration

Authors - Jennifer Kosinski
View Article
chickenWB

Microbiol Spectr

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

Authors - Beibei Zhang
  • KD Validated
View Article
mouseIF

Mol Neurobiol

Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling.

Authors - Qingxiu Yao
View Article
ratWB

Front Pharmacol

Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca2+ Signaling Pathway.

Authors - Lei Liu
View Article