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CoraLite® Plus 750-conjugated Talin-1 Polyclonal antibody
Talin-1 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 750 Fluorescent Dye
Cat no : CL750-14168
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL750-14168 targets Talin-1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Talin-1 fusion protein Ag5367 相同性解析による交差性が予測される生物種 |
Full Name | talin 1 |
Calculated molecular weight | 2541 aa, 270 kDa |
Observed molecular weight | 250-270 kDa |
GenBank accession number | BC042923 |
Gene symbol | Talin-1 |
Gene ID (NCBI) | 7094 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 750 Talin-1 antibody CL750-14168 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |