• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TLR3 Recombinant antibody, PBS Only

TLR3 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230445G4

Cat no : 83136-3-PBS

Synonyms

Toll-like receptor 3, toll like receptor 3, CD283, 230445G4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TLR3 Recombinant antibody (83136-3-RR)

    WB analysis using 83136-3-RR (same clone as 83136-3-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using TLR3 Recombinant antibody (83136-3-RR)

    IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using TLR3 Recombinant antibody (83136-3-RR)

    IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TLR3 Recombinant antibody (83136-3-RR)

    IHC staining of human placenta using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TLR3 Recombinant antibody (83136-3-RR)

    IHC staining of human placenta using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83136-3-RR

    Affinity and Kinetic Characterization of 83136-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83136-3-PBS targets TLR3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TLR3 fusion protein Ag34044 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 3
Calculated molecular weight 904 aa, 104 kDa
Observed molecular weight120 kDa, 68 kDa
GenBank accession numberBC094737
Gene symbol TLR3
Gene ID (NCBI) 7098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).