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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TLR3 Recombinant antibody

TLR3 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230445G4

Cat no : 83136-3-RR

Synonyms

Toll-like receptor 3, toll like receptor 3, CD283, 230445G4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TLR3 Recombinant antibody (83136-3-RR)

    WB analysis using 83136-3-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using TLR3 Recombinant antibody (83136-3-RR)

    IHC staining of human ovarian cancer using 83136-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovarian cancer using TLR3 Recombinant antibody (83136-3-RR)

    IHC staining of human ovarian cancer using 83136-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TLR3 Recombinant antibody (83136-3-RR)

    IHC staining of human placenta using 83136-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TLR3 Recombinant antibody (83136-3-RR)

    IHC staining of human placenta using 83136-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83136-3-RR

    Affinity and Kinetic Characterization of 83136-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHT-29 cells, human placenta tissue
Positive IHC detected inhuman ovarian cancer, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83136-3-RR targets TLR3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TLR3 fusion protein Ag34044 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 3
Calculated molecular weight 904 aa, 104 kDa
Observed molecular weight120 kDa, 68 kDa
GenBank accession numberBC094737
Gene symbol TLR3
Gene ID (NCBI) 7098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).

Protocols

Product Specific Protocols
WB protocol for TLR3 antibody 83136-3-RRDownload protocol
IHC protocol for TLR3 antibody 83136-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols