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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody

TLR4/CD284 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

230463C7

Cat no : 98018-1-RR

Synonyms

Tlr4, CD284, toll like receptor 4, 230463C7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Balb/c mouse peritoneal macrophages using Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR)

    FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.

  • Flow cytometry (FC) experiment of Balb/c mouse peritoneal macrophages using Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR)

    FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

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Tested Applications

Positive FC detected inBalb/c mouse peritoneal macrophages

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98018-1-RR is TLR4/CD284 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TLR4/CD284 fusion protein Ag32998 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 4
Calculated molecular weight96 kDa
GenBank accession numberNM_021297
Gene symbol Tlr4
Gene ID (NCBI) 21898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

Protocols

Product Specific Protocols
FC protocol for TLR4/CD284 antibody 98018-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols