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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody

TLR4/CD284 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230463C7

Cat no : CL647-98018

Synonyms

Tlr4, CD284, toll like receptor 4, 230463C7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Balb/c mouse peritoneal macrophages using CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Re (CL647-98018)

    FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with FITC Plus Anti-Mouse CD11b and 0.25 ug CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (CL647-98018, Clone: 230463C7) or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of Balb/c mouse peritoneal macrophages using CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Re (CL647-98018)

    FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (CL647-98018, Clone: 230463C7) (red) or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inBalb/c mouse peritoneal macrophages

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98018 is TLR4/CD284 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TLR4/CD284 fusion protein Ag32998 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 4
Calculated molecular weight96 kDa
GenBank accession numberNM_021297
Gene symbol Tlr4
Gene ID (NCBI) 21898
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 TLR4/CD284 antibody CL647-98018Download protocol
Standard Protocols
Click here to view our Standard Protocols