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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TLR5 Monoclonal antibody

TLR5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4B3D2

Cat no : 66570-1-Ig

Synonyms

Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using TLR5 Monoclonal antibody (66570-1-Ig)

    WB analysis of A549 using 66570-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using TLR5 Monoclonal antibody (66570-1-Ig)

    WB analysis of THP-1 using 66570-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using TLR5 Monoclonal antibody (66570-1-Ig)

    WB analysis of RAW 264.7 using 66570-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using TLR5 Monoclonal antibody (66570-1-Ig)

    WB analysis of rat spleen using 66570-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TLR5 Monoclonal antibody (66570-1-Ig)

    WB analysis of HeLa using 66570-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using TLR5 Monoclonal antibody (66570-1-Ig)

    IF Staining of THP-1 using 66570-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using TLR5 Monoclonal antibody (66570-1-Ig)

    IF Staining of THP-1 using 66570-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of THP-1 cells using TLR5 Monoclonal antibody (66570-1-Ig)

    FC experiment of THP-1 using 66570-1-Ig

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TLR5 (66570-1-Ig, Clone:4B3D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA549 cells, THP-1 cells, rat spleen tissue, HeLa cells, RAW 264.7 cells
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66570-1-Ig targets TLR5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TLR5 fusion protein Ag13840 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 5
Calculated molecular weight 858 aa, 98 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC109119
Gene symbol TLR5
Gene ID (NCBI) 7100
RRIDAB_2881931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Protocols

Product Specific Protocols
WB protocol for TLR5 antibody 66570-1-IgDownload protocol
IF protocol for TLR5 antibody 66570-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nutr Diabetes

Gestational diabetes exacerbates intrauterine microbial exposure induced intestinal microbiota change in offspring contributing to increased immune response

Authors - Juncheng Liu
View Article