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Validation Data Gallery

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  • Flow cytometry (FC) experiment of THP-1 cells using CoraLite® Plus 488-conjugated TLR5 Monoclonal anti (CL488-66570)

    FC experiment of THP-1 using CL488-66570

    1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TLR5 (CL488-66570, Clone:4B3D2) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated TLR5 Monoclonal antibody

TLR5 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66570
Clone No.4B3D2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

FC (Intra)

Synonyms

Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66570 targets TLR5 in FC (Intra) applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TLR5 fusion protein Ag13840 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 5
Calculated molecular weight 858 aa, 98 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC109119
Gene Symbol TLR5
Gene ID (NCBI) 7100
RRIDAB_3084259
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60602
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TLR5 antibody CL488-66570Download protocol
Standard Protocols
Click here to view our Standard Protocols