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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TLR8 Monoclonal antibody, PBS Only

TLR8 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B6A4

Cat no : 67317-1-PBS

Synonyms

CD288, TLR8, toll like receptor 8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TLR8 Monoclonal antibody (67317-1-Ig)

    WB analysis using 67317-1-Ig (same clone as 67317-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using TLR8 Monoclonal antibody (67317-1-Ig)

    WB analysis of U-937 using 67317-1-Ig (same clone as 67317-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TLR8 Monoclonal antibody (67317-1-Ig)

    IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TLR8 Monoclonal antibody (67317-1-Ig)

    IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TLR8 Monoclonal antibody (67317-1-Ig)

    IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TLR8 Monoclonal antibody (67317-1-Ig)

    IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67317-1-PBS targets TLR8 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TLR8 fusion protein Ag16566 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 8
Calculated molecular weight 1041 aa, 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC101075
Gene symbol TLR8
Gene ID (NCBI) 51311
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.